{
  "url": "https://openlegislation.uk/eu-regulation-2008-900/annex-I/division-6/division-6.4/division-6.4.1",
  "json_url": "https://openlegislation.uk/eu-regulation-2008-900/annex-I/division-6/division-6.4/division-6.4.1.json",
  "legislation": "eur/2008/900",
  "legislation_title": "Commission Regulation (EC) No 900/2008 of 16 September 2008 laying down the methods of analysis and other technical provisions necessary for the application of the arrangements for imports of certain goods resulting from the processing of agricultural products (Codified version)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2008-900",
  "path": "annex/I/division/6/division/6.4/division/6.4.1",
  "kind": "division",
  "number": "6.4.1",
  "heading": "Preparation of the samples",
  "text": "Homogenise the sample by shaking or stirring. The chosen test portion (point 6.2) is weighed into a volumetric flask (point 5.5) and about 70 ml warm water is added.\nAfter dissolving or suspending, add 50 microliter of thermostable alpha-amylase (point 4.6) and heat at 90 °C for 30 min in a water bath (point 5.9). Cool as quick as possible to 60 °C in a water bath, and add 5 ml of a solution of amyloglucosidase (point 4.13). For samples which could influence the pH of the reaction solution, control the pH and adjust it to 4,6 to 4,8, if necessary. Allow to react for 60 min at 60 °C. Cool the samples to ambient temperature.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Commission Regulation (EU) No 118/2010 of 9 February 2010 amending Regulation (EC) No 900/2008 laying down the methods of analysis and other technical provisions necessary for the application of the arrangements for imports of certain goods resulting from the processing of agricultural products."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2008/900/annex/I/division/6/division/6.4/division/6.4.1",
    "retrieved_at": "2026-11-01T14:52:01.805747+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}