# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 5.2.1: Distillation into sulphuric acid

Extent: E+W+S+N.I.

Place in the collecting flask of the distillation apparatus an exactly measured quantity of 25 ml of sulphuric acid (3.5) or (3.7) depending on the presumed nitrogen content. Add a few drops of methyl red indicator (3.8).
Connect the digestion flask to the condenser of the distillation apparatus and immerse the end of the condenser in the liquid contained in the collecting flask to a depth of at least 1 cm (see observation 8.3). Slowly pour 100 ml of sodium hydroxide solution (3.9) into the digestion flask without loss of ammonia (see observation 8.1). Heat the flask until the ammonia has distilled over.

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/III/division/C/division/5/division/5.2/division/5.2.1 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-C/division-5/division-5.2/division-5.2.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.