# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 6.2.2: Titration with sulphuric acid

Extent: E+W+S+N.I.

The content of crude protein, expressed as a percentage by weight, is calculated according to the following formula:
V1−V0×c×2,8×6,25mwhere:
mis the weight (g) of the test portion,
cis the concentration (mol/l) of the standard volumetric solution of sulphuric acid (3.6),
V0 is the volume (in ml) of sulphuric acid (3.6) used for the blank test,
V1 is the volume (in ml) of sulphuric acid (3.6) used for test portion.

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/III/division/C/division/6/division/6.2/division/6.2.2 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-C/division-6/division-6.2/division-6.2.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.