# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 2: Principle

Extent: E+W+S+N.I.

The sample is extracted with water and the solution clarified and filtered. The volatile nitrogenous bases are displaced at boiling point by adding magnesium oxide and collected in a specific quantity of sulphuric acid, the excess of which is back-titrated with a solution of sodium hydroxide.

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/III/division/E/division/II/division/2 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-E/division-II/division-2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.