# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 2.1: Free amino acids

Extent: E+W+S+N.I.

The free amino acids are extracted with diluted hydrochloric acid. Co-extracted nitrogenous macromolecules are precipitated with sulfosalicylic acid and removed by filtration. The filtered solution is adjusted to pH 2,2. The amino acids are separated by ion exchange chromatography and determined by reaction with ninhydrin with photometric detection at 570 nm.

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/III/division/F/division/2/division/2.1 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-F/division-2/division-2.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.