{
  "url": "https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-F/division-3/division-3.27.4",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-F/division-3/division-3.27.4.json",
  "legislation": "eur/2009/152",
  "legislation_title": "Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-152",
  "path": "annex/III/division/F/division/3/division/3.27.4",
  "kind": "division",
  "number": "3.27.4",
  "heading": "Calibration solution of standard amino acids for use with hydrolysates, c = 5 nmol/50 μl of cysteic acid and methionine sulphone and c = 10 nmol/50 μl of the other amino acids. Dissolve 2,2 g sodium chloride (3.10) in 100 ml beaker with 30 ml citrate buffer (3.24). Add 4,0 ml stock standard solution of amino acids (3.27.1), 4,0 ml stock standard solution of cysteic acid and methionine sulphone (3.27.2) and 0,5 ml stock standard solution of internal standard (3.27.3) if used. Adjust pH to 2,2 with sodium hydroxide (3.18).",
  "text": "Transfer quantitatively to a 50 ml graduated flask and make up to the mark with citrate buffer (3.24) and mix.\nStore below 5 oC for not more than 3 months.\nSee also observation 9.1.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/152/annex/III/division/F/division/3/division/3.27.4",
    "retrieved_at": "2026-11-01T14:51:38.610873+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}