{
  "url": "https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-F/division-5/division-5.3/division-5.3.1",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-F/division-5/division-5.3/division-5.3.1.json",
  "legislation": "eur/2009/152",
  "legislation_title": "Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-152",
  "path": "annex/III/division/F/division/5/division/5.3/division/5.3.1",
  "kind": "division",
  "number": "5.3.1",
  "heading": "Oxidation",
  "text": "Weigh to the nearest 0,2 mg from 0,1 to 1 g of the prepared sample (5.1) into:\na 100 ml round-bottomed flask (4.1) for open hydrolysis (5.3.2.3) or,\na 250 ml round-bottomed flask (4.1) if a low sodium concentration is required (5.3.3.1) or,\na 100 ml bottle fitted with a screw cap (4.2), for closed hydrolysis (5.3.2.4).\nThe weighed sample portion must have a nitrogen content of about 10 mg and a moisture content not exceeding 100 mg.\nPlace the flask/bottle in an ice-water bath and cool to 0 oC, add 5 ml of oxidation mixture (3.23) and mix using a glass spatula with a bent tip. Seal the flask/bottle containing the spatula with an air-tight film, place the ice-water bath containing the sealed container in a refrigerator at 0 oC and leave for 16 hours. After 16 hours remove from the refrigerator and decompose the excess oxidation reagent by the addition of 0,84 g of sodium disulphite (3.4).\nProceed to 5.3.2.1.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/152/annex/III/division/F/division/5/division/5.3/division/5.3.1",
    "retrieved_at": "2026-11-01T14:51:38.610873+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}