# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 5.3.2.2: Hydrolysis of unoxidised samples

Extent: E+W+S+N.I.

Weigh into either a 100 ml or a 250 ml round-bottom flask (4.1) or a 100 ml bottle fitted with a screw cap (4.2), to the nearest 0,2 mg, from 0,1 to 1 g of the prepared sample (5.1). The weighed sample portion must have a nitrogen content of about 10 mg. Add carefully 25 ml of hydrolysis mixture (3.20) and mix with the sample. Proceed according to either 5.3.2.3 or 5.3.2.4.

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/III/division/F/division/5/division/5.3/division/5.3.2/division/5.3.2.2 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-F/division-5/division-5.3/division-5.3.2/division-5.3.2.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.