{
  "url": "https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-F/division-5/division-5.3/division-5.3.2/division-5.3.2.3",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-F/division-5/division-5.3/division-5.3.2/division-5.3.2.3.json",
  "legislation": "eur/2009/152",
  "legislation_title": "Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-152",
  "path": "annex/III/division/F/division/5/division/5.3/division/5.3.2/division/5.3.2.3",
  "kind": "division",
  "number": "5.3.2.3",
  "heading": "Open hydrolysis",
  "text": "Add 3 glass beads to the mixture in the flask (prepared in accordance with 5.3.2.1 or 5.3.2.2) and boil with continuous bubbling under reflux for 23 hours. On completion of hydrolysis, wash the condenser down with 5 ml of citrate buffer (3.24). Disconnect the flask and cool it in an ice bath.\nProceed according to 5.3.3.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/152/annex/III/division/F/division/5/division/5.3/division/5.3.2/division/5.3.2.3",
    "retrieved_at": "2026-11-01T14:51:38.610873+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}