# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 5.3.4: Sample solution for chromatography

Extent: E+W+S+N.I.

Quantitatively transfer the pH adjusted hydrolysate (5.3.3.1 or 5.3.3.2) with citrate buffer (3.24) to a 200 ml graduated flask, and make up to the mark with buffer (3.24).
If an internal standard has not already been used, add 2,0 ml of internal standard (3.27.3) and make up to the mark with citrate buffer (3.24). Mix thoroughly.
Proceed to the chromatography step (5.4).
If the sample solutions are not being examined the same day they must be stored below 5 oC.

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/III/division/F/division/5/division/5.3/division/5.3.4 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-F/division-5/division-5.3/division-5.3.4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.