{
  "url": "https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-F/division-6",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-F/division-6.json",
  "legislation": "eur/2009/152",
  "legislation_title": "Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-152",
  "path": "annex/III/division/F/division/6",
  "kind": "division",
  "number": "6",
  "heading": "Calculation of results",
  "text": "The area of the sample and standard peaks is measured for each individual amino acid and the amount (X), in g amino acid per kg sample, is calculated as follows:\nX=A×c×M×VB×m×1 000If an internal standard is used multiply by: DC\nApeak area, hydrolysate or extract\nBpeak area, calibration standard solution\nCpeak area, internal standard in hydrolysate or extract\nDpeak area, internal standard, calibration standard solution\nMmolar weight of the amino acid being determined\ncconcentration of standard in μmol/ml\nmsample weight (g) (corrected to original weight if dried or defatted)\nVml total hydrolysate (5.3.4) or ml calculated total dilution volume of extract (6.1)\nCystine and cysteine are both determined as cysteic acid in hydrolysates of oxidised sample, but calculated as cystine (C6H12N2O4S2, M 240,3 g/mol) by using M 120,15 g/mol (= 0,5 x 240,3 g/mol).\nMethionine is determined as methionine sulphone in hydrolysates of oxidised sample, but calculated as methionine by using M of methionine: 149,21 g/mol.\nAdded free methionine is determined after extraction as methionine, for the calculation the same M is used.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/152/annex/III/division/F/division/6",
    "retrieved_at": "2026-11-01T14:51:38.610873+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}