# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 6.1: The total dilution volume of extracts (F) for determination of free amino acids (5.2) is calculated as follows:

Extent: E+W+S+N.I.

F=100 ml×10 ml+5 ml10 ml×V10VVolume of final extract

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/III/division/F/division/6/division/6.1 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-F/division-6/division-6.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.