# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 3.22

Extent: E+W+S+N.I.

Mobile phase for HPLC: 3,0 g acetic acid (3.18) + 900 ml water (3.1) +50,0 ml solution (3.21) of 1,1,1-trichloro-2-methyl-2-propanol (3.19) in methanol (3.8) (1g/100ml). Adjust pH to 5,0 using ethanolamine (3.20). Make up to 1 000 ml with water (3.1).

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/III/division/G/division/3/division/3.22 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-G/division-3/division-3.22
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.