{
  "url": "https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-G/division-5/division-5.4",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-G/division-5/division-5.4.json",
  "legislation": "eur/2009/152",
  "legislation_title": "Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-152",
  "path": "annex/III/division/G/division/5/division/5.4",
  "kind": "division",
  "number": "5.4",
  "heading": "HPLC determination",
  "text": "The following conditions for isocratic elution are offered for guidance; other conditions may be used, provided they yield equivalent results (see also observations 9.1 and 9.2):\nLiquid chromatographic column (4.2):\n125 mm x 4 mm, C18, 3 μm packing or equivalent\nColumn temperature:\nRoom temperature\nMobile phase (3.22):\n3,0 g acetic acid (3.18) + 900 ml water (3.1) +50,0 ml solution (3.21) of 1,1,1-trichloro-2- methyl-2-propanol (3.19) in methanol (3.8) (1 g/100 ml). Adjust pH to 5,0 using ethanolamine (3.20). Make up to 1 000 ml with water (3.1)\nFlow rate:\n1 ml/min.\nTotal run time:\napprox. 34 min.\nDetection wavelength:\nexcitation: 280 nm, emission: 356 nm.\nInjection volume\n20 μl",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/152/annex/III/division/G/division/5/division/5.4",
    "retrieved_at": "2026-11-01T14:51:38.610873+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}