{
  "url": "https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-G/division-6",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-G/division-6.json",
  "legislation": "eur/2009/152",
  "legislation_title": "Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-152",
  "path": "annex/III/division/G/division/6",
  "kind": "division",
  "number": "6",
  "heading": "Calculation of results",
  "text": "The amount of tryptophane (X), in g per 100g sample, is calculated as follows:\nX=A×B×V1×c×V2×MC×D×V3×10 000×mApeak area of internal standard, calibration standard solution (3.17)\nBpeak area of tryptophan, extract (5.2) or hydrolysate (5.3)\nV1 volume in ml (2 ml) of concentrated tryptophan solution (3.15) added to the calibration solution (3.17)\ncconcentration in μmol/ml (= 2,5) of concentrated tryptophan solution (3.15) added to calibration solution (3.17)\nV2 volume in ml of concentrated internal standard solution (3.16) added at the extraction (5.2) (= 5,0 ml) or to the hydrolysate (5.3) (= 2,0 ml)\nCpeak area of internal standard, extract (5.2) or hydrolysate (5.3)\nDpeak area of tryptophan, calibration standard solution (3.17)\nV3 volume in ml (= 2,0 ml) of concentrated internal standard solution (3.16) added to calibration standard solution (3.17)\nmsample weight in g (corrected to original weight if dried and/or defatted)\nMmolar weight of tryptophan (= 204,23 g/mol)",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/152/annex/III/division/G/division/6",
    "retrieved_at": "2026-11-01T14:51:38.610873+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}