{
  "url": "https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-G/division-9/division-9.1",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-152/annex-III/division-G/division-9/division-9.1.json",
  "legislation": "eur/2009/152",
  "legislation_title": "Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-152",
  "path": "annex/III/division/G/division/9/division/9.1",
  "kind": "division",
  "number": "9.1",
  "heading": "Following special chromatographic conditions may give better separation between tryptophan and α-methyl-tryptophan.",
  "text": "Isocratic elution followed by gradient column cleaning:\nLiquid chromatographic column:\n125 mm x 4 mm, C18, 5 μm packing or equivalent\nColumn temperature:\n32 oC\nMobile phase:\nA: 0,01 mol/l KH2PO4/méthanol, 95+5 (V+V).\nB: methanol\nGradient program:\n0 min.\n100 % A\n0 % B\n15 min.\n100 % A\n0 % B\n17 min.\n60 % A\n40 % B\n19 min.\n60 % A\n40 % B\n21 min.\n100 % A\n0 % B\n33 min.\n100 % A\n0 % B\nFlow rate:\n1,2 ml/min.\nTotal run time:\napprox. 33 min.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/152/annex/III/division/G/division/9/division/9.1",
    "retrieved_at": "2026-11-01T14:51:38.610873+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}