Division 1 Purpose and Scope

This method makes it possible to determine the level of vitamin A (retinol) in feed and premixtures. Vitamin A includes all-trans-retinyl alcohol and its cis-isomers which are determined by this method. The content of vitamin A is expressed in International Units (IU) per kg. One IU corresponds to the activity of 0,3 μg all-trans-vitamin A alcohol or 0,344 μg all-trans-vitamin A acetate or 0,55 μg all-trans-vitamin A palmitate.

The limit of quantification is 2 000 IU vitamin A/kg.