# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division: HPLC conditions

Extent: E+W+S+N.I.

The following conditions are offered for guidance; other conditions may be used provided that they give equivalent results.
Liquid chromatographic column (4.5.1):
250 mm × 4 mm, C18, 5 or 10 μm packing, or equivalent
Mobile phase (3.9):
Mixture of methanol (3.3) and water e.g. 980 + 20 (v + v).
Flow rate:
1-2 ml/min.
Detector (4.5.2):
UV detector (325 nm) or fluorescence detector
(excitation: 325 nm/emission: 475 nm)

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/IV/division/A/division/5/division/5.5/division/1 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-IV/division-A/division-5/division-5.5/division-1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.