# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 5.6.2: Preparation of the calibration solutions and calibration graph

Extent: E+W+S+N.I.

Transfer 1,0, 2,0, 5,0 and 10,0 ml of the diluted working standard solution into a series of 20 ml graduated flasks, make up to the mark with methanol (3.3) and mix. The nominal concentrations of these solutions are 2,8, 5,6, 14,0 and 28,0 IU vitamin A per ml.
Inject 20 μl of each calibration solution several times and determine the mean peak heights (areas). Using the mean peak heights (areas) plot a calibration graph considering the results of the UV control (5.6.3.3).

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/IV/division/A/division/5/division/5.6/division/5.6.2 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-IV/division-A/division-5/division-5.6/division-5.6.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.