# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 7.9: In cases of analysis of vitamin A in milk replacers, specific attention has to be paid

Extent: E+W+S+N.I.

at saponification (5.2): due to the amount of fat present in the sample, increasing of potassium hydroxide solution amount (3.4) may be necessary,
at extraction (5.3): due to the presence of emulsions, adaptation of the water/ethanol 2:1 ratio may be necessary.
To check if the applied method of analysis generates reliable results on this specific matrix (milk replacer), a recovery test shall be applied on an additional test portion. If the recovery rate is lower than 80 %, the analytical result has to be corrected for recovery.

---
Source: https://www.legislation.gov.uk/eur/2009/152/annex/IV/division/A/division/7/division/7.9 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-IV/division-A/division-7/division-7.9
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.