{
  "url": "https://openlegislation.uk/eu-regulation-2009-152/annex-IV/division-D/division-5/division-5.3/division-5.3.1",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-152/annex-IV/division-D/division-5/division-5.3/division-5.3.1.json",
  "legislation": "eur/2009/152",
  "legislation_title": "Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-152",
  "path": "annex/IV/division/D/division/5/division/5.3/division/5.3.1",
  "kind": "division",
  "number": "5.3.1",
  "heading": "Preparation of the Amberlite column",
  "text": "An XAD-2 column is prepared for each sample extract. Transfer 10 g of prepared Amberlite (3.19) into a glass column (4.5) with methanol (3.8). Add a small plug of glass-wool to the top of the resin bed. Drain the methanol from the column and wash the resin with 100 ml of water, stopping the flow as the liquid reaches the top of the resin bed. Allow the column to equilibrate for 10 minutes before use. Never allow the column to run dry.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/152/annex/IV/division/D/division/5/division/5.3/division/5.3.1",
    "retrieved_at": "2026-11-01T14:51:38.610873+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}