# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 3.8: HPLC mobile phase.

Extent: E+W+S+N.I.

Mix together the following reagents:
650 ml acetonitrile (3.3),
250 ml water (equivalent to HPLC-grade),
50 ml potassium di-hydrogen phosphate solution (3.6),
50 ml di-sodium hydrogen phosphate solution (3.7).
Filter through a 0,22 μm filter (4.6) and degas the solution, (e.g. by ultrasonification for 10 minutes).

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/IV/division/E/division/3/division/3.8 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-IV/division-E/division-3/division-3.8
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.