# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 5.4.1: Parameters

Extent: E+W+S+N.I.

The following conditions are offered for guidance, other conditions may be used provided they yield equivalent results:
Liquid chromatographic column (4.4.1),
HPLC mobile phase (3.8),
Flow rate: 1,5 to 2 ml/minute,
Detector wavelength: 317 nm,
Injection volume: 20 to 50 μl.
Check the stability of the chromatographic system, injecting the calibration solution (3.9.3) containing 3,6 μg/ml several times, until constant peak heights and retention times are achieved.

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/IV/division/E/division/5/division/5.4/division/5.4.1 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-IV/division-E/division-5/division-5.4/division-5.4.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.