{
  "url": "https://openlegislation.uk/eu-regulation-2009-152/annex-IV/division-F/division-5/division-5.3/division-5.3.1",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-152/annex-IV/division-F/division-5/division-5.3/division-5.3.1.json",
  "legislation": "eur/2009/152",
  "legislation_title": "Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-152",
  "path": "annex/IV/division/F/division/5/division/5.3/division/5.3.1",
  "kind": "division",
  "number": "5.3.1",
  "heading": "Parameters",
  "text": "The following conditions are offered for guidance, other conditions may be used provided that they give equivalent results.\nLiquid chromatographic column (4.2.1)\n100 mm × 4,6 mm, Hypersil ODS, 3 μm packing, or equivalent\nMobile phase:\nEluent A (3.13.1):\nAqueous solution of ammonium acetate and tetrabutyl-ammonium hydrogen sulphate\nEluent B (3.13.2):\nacetonitrile\nEluent C (3.13.3):\nmethanol\nElution mode:\nlinear gradient\ninitial conditions: A + B + C = 60 + 20 + 20 (V + V + V)\nafter 10 min. gradient elution during 30 min. to: A + B + C = 45 + 20 + 35 (V + V + V)\nFlush with B during 10 min.\nFlow rate:\n1,5-2 ml/min.\nInjection volume:\n20 μl\nDetector wavelength:\n280 nm.\nCheck the stability of the chromatographic system, injecting several times the calibration solution (3.10), containing 2 μg/ml, until constant peak heights and retention times are achieved.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/152/annex/IV/division/F/division/5/division/5.3/division/5.3.1",
    "retrieved_at": "2026-11-01T14:51:38.610873+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}