{
  "url": "https://openlegislation.uk/eu-regulation-2009-152/annex-IV/division-G/division-5/division-5.3/division-5.3.1",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-152/annex-IV/division-G/division-5/division-5.3/division-5.3.1.json",
  "legislation": "eur/2009/152",
  "legislation_title": "Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-152",
  "path": "annex/IV/division/G/division/5/division/5.3/division/5.3.1",
  "kind": "division",
  "number": "5.3.1",
  "heading": "Parameters",
  "text": "The following conditions are offered for guidance; other conditions may be used, provided they yield equivalent results:\nLiquid chromatographic column (4.3.1):\n125 mm × 4 mm, reversed-phase C18, 5 μm packing or equivalent\nMobile phase (3.9):\nMixture of phosphate buffer solution (3.7) and methanol (3.5), 5+95 (V+V)\nFlow rate:\n1,2 ml/min.\nDetection wavelengths:\nExcitation:\n310 nm\nEmission:\n419 nm\nInjection volume:\n20 μl\nCheck the stability of the chromatographic system, injecting the calibration solution (3.10.3) containing 4,0 μg/ml several times, until constant peak heights (or areas) and retention times are achieved.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/152/annex/IV/division/G/division/5/division/5.3/division/5.3.1",
    "retrieved_at": "2026-11-01T14:51:38.610873+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}