# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 5.3.1: Parameters

Extent: E+W+S+N.I.

The following conditions are offered for guidance; other conditions may be used, provided they yield equivalent results:
Liquid chromatographic column (4.3.1):
125 mm × 4 mm, reversed-phase C18, 5 μm packing or equivalent
Mobile phase (3.9):
Mixture of phosphate buffer solution (3.7) and methanol (3.5), 5+95 (V+V)
Flow rate:
1,2 ml/min.
Detection wavelengths:
Excitation:
310 nm
Emission:
419 nm
Injection volume:
20 μl
Check the stability of the chromatographic system, injecting the calibration solution (3.10.3) containing 4,0 μg/ml several times, until constant peak heights (or areas) and retention times are achieved.

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/IV/division/G/division/5/division/5.3/division/5.3.1 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-IV/division-G/division-5/division-5.3/division-5.3.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.