# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 7.3.3

Extent: E+W+S+N.I.

Calculation as mean value of bioanalytical results (in BEQ, corrected for blank and recovery) from multiple analysis of samples (n ≥ 6) contaminated at two-thirds of the maximum level or action threshold, based on the observation that this level will be around the cut-off value determined under point 7.3.1 or point 7.3.2:
Calculation of cut-off values based on a 95 % level of confidence implying a false-compliant rate < 5 %, and a RSD R < 25 %:
(1)from the lower band of the 95 % prediction interval at the decision limit of the confirmatory method.
(2)from multiple analysis of samples (n ≥ 6) contaminated at the decision limit of the confirmatory method as the lower end-point of the data distribution (represented in the figure by a bell-shaped curve) at the corresponding mean BEQ value.

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/V/division/B/chapter/II/division/7/division/7.3/division/7.3.3 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-V/division-B/chapter-II/division-7/division-7.3/division-7.3.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.