{
  "url": "https://openlegislation.uk/eu-regulation-2009-152/annex-VI/division-2/division-2.1/division-2.1.3/division-2.1.3.4",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-152/annex-VI/division-2/division-2.1/division-2.1.3/division-2.1.3.4.json",
  "legislation": "eur/2009/152",
  "legislation_title": "Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-152",
  "path": "annex/VI/division/2/division/2.1/division/2.1.3/division/2.1.3.4",
  "kind": "division",
  "number": "2.1.3.4",
  "heading": "Preparation of samples consisting of fat or oil",
  "text": "The following protocol shall be followed for the preparation of samples consisting of fat or oil:\nif the fat is solid, it shall be warmed in a oven until it is liquid.\nby using a pipette, 40 ml of fat or oil shall be transferred from the bottom of the sample to a centrifugation tube.\ncentrifuge during 10 minutes at 4 000 r.p.m.\nif the fat is solid after centrifugation, it shall be warmed in an oven until it is liquid.\nrepeat the centrifugation during 5 minutes at 4 000 r.p.m.\nby using a small spoon or a spatula, one half of the decanted impurities shall be transferred to microscopic slides for examination, Glycerol is recommended as mounting medium.\nthe remaining impurities shall be used for preparing the sediment as described in point 2.1.3.3.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Commission Regulation (EU) No 51/2013 of 16 January 2013 amending Regulation (EC) No 152/2009 as regards the methods of analysis for the determination of constituents of animal origin for the official control of feed (Text with EEA relevance)."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/152/annex/VI/division/2/division/2.1/division/2.1.3/division/2.1.3.4",
    "retrieved_at": "2026-11-01T14:51:38.610873+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}