# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 2.1.3.4: Preparation of samples consisting of fat or oil

Extent: E+W+S+N.I.

The following protocol shall be followed for the preparation of samples consisting of fat or oil:
if the fat is solid, it shall be warmed in a oven until it is liquid.
by using a pipette, 40 ml of fat or oil shall be transferred from the bottom of the sample to a centrifugation tube.
centrifuge during 10 minutes at 4 000 r.p.m.
if the fat is solid after centrifugation, it shall be warmed in an oven until it is liquid.
repeat the centrifugation during 5 minutes at 4 000 r.p.m.
by using a small spoon or a spatula, one half of the decanted impurities shall be transferred to microscopic slides for examination, Glycerol is recommended as mounting medium.
the remaining impurities shall be used for preparing the sediment as described in point 2.1.3.3.

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/VI/division/2/division/2.1/division/2.1.3/division/2.1.3.4 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-VI/division-2/division-2.1/division-2.1.3/division-2.1.3.4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.