{
  "url": "https://openlegislation.uk/eu-regulation-2009-152/annex-VI/division-2/division-2.2/division-2.2.5",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-152/annex-VI/division-2/division-2.2/division-2.2.5.json",
  "legislation": "eur/2009/152",
  "legislation_title": "Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-152",
  "path": "annex/VI/division/2/division/2.2/division/2.2.5",
  "kind": "division",
  "number": "2.2.5",
  "heading": "Genetic amplification",
  "text": "The genetic amplification shall be performed using the methods validated for each species requiring identification. These methods are laid down in the SOP established by the EURL-AP and published on its website. Each DNA extract shall be analysed at least at two different dilutions in order to evaluate inhibition.\nTwo amplification controls shall be prepared per species target as described by ISO 24276.\na positive DNA target control shall be used for each plate or series of PCR assays,\nan amplification reagent control (also called no template control) shall be used for each plate or series of PCR assays.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Commission Regulation (EU) No 51/2013 of 16 January 2013 amending Regulation (EC) No 152/2009 as regards the methods of analysis for the determination of constituents of animal origin for the official control of feed (Text with EEA relevance)."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/152/annex/VI/division/2/division/2.2/division/2.2.5",
    "retrieved_at": "2026-11-01T14:51:38.610873+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}