# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 3.3: HPLC mobile phase

Extent: E+W+S+N.I.

Mixture of methanol (3.2) and water (equivalent to HPLC grade) 75 + 25 (v + v).
Filter through a 0,22 μm filter (4.5) and degas the solution (e.g. by ultrasonification for 10 minutes).

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/VIII/division/A/division/3/division/3.3 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-VIII/division-A/division-3/division-3.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.