# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 5.3.1: Parameters:

Extent: E+W+S+N.I.

The following conditions are offered for guidance, other conditions may be used provided that they give equivalent results.
Analytical column (4.3.1)
Mobile Phase (3.4):
water (3.3)-methanol (3.2) mixture, 900 + 100 (V + V)
Flow rate:
1,5-2 ml/min.
Detection wavelength:
380 nm
Injection volume:
20 μl –100 μl
Check the stability of the chromatographic system, injecting several times the calibration solution (3.5.3) containing 2,5 μg/ml, until constant peak heights and retention times are achieved.

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/VIII/division/B/division/5/division/5.3/division/5.3.1 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-VIII/division-B/division-5/division-5.3/division-5.3.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.