# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 3.6: Mobile phase for HPLC (see observation 9.1).

Extent: E+W+S+N.I.

Mixture of acetonitrile (3.2), sodium dihydrogen phosphate solution (3.4) and sodium perchlorate solution (3.5), 450+450+100 (v+v+v). Prior to use filter through a 0,22 μm membrane filter (4.3) and degas the solution (e.g. in the ultrasonic bath (4.4) for at least 15 minutes).

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/VIII/division/C/division/3/division/3.6 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-VIII/division-C/division-3/division-3.6
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.