{
  "url": "https://openlegislation.uk/eu-regulation-2009-152/annex-VIII/division-C/division-5/division-5.3/division-5.3.1",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-152/annex-VIII/division-C/division-5/division-5.3/division-5.3.1.json",
  "legislation": "eur/2009/152",
  "legislation_title": "Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-152",
  "path": "annex/VIII/division/C/division/5/division/5.3/division/5.3.1",
  "kind": "division",
  "number": "5.3.1",
  "heading": "Parameters:",
  "text": "The following conditions are offered for guidance, other conditions may be used provided that they give equivalent results.\nLiquid chromatographic\ncolumn (4.1.1):\n125 mm × 4 mm, cation exchange Nucleosil 10 SA, 5 or 10 μm packing, or equivalent\nMobile phase (3.6):\nMixture of acetonitrile (3.2), sodium dihydrogen phosphate solution (3.4) and sodium perchlorate solution (3.5), 450+450+100 (v+v+v).\nFlow rate:\n0,7-1 ml/min\nDetection wavelength:\n264 nm\nInjection volume:\n100 μl\nCheck the stability of the chromatographic system, injecting several times the calibration solution (3.7.3) containing 1,0 μg/ml, until constant peak heights and retention times are achieved.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/152/annex/VIII/division/C/division/5/division/5.3/division/5.3.1",
    "retrieved_at": "2026-11-01T14:51:38.610873+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}