# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 5.3.1: Parameters:

Extent: E+W+S+N.I.

The following conditions are offered for guidance, other conditions may be used provided that they give equivalent results.
Liquid chromatographic
column (4.1.1):
125 mm × 4 mm, cation exchange Nucleosil 10 SA, 5 or 10 μm packing, or equivalent
Mobile phase (3.6):
Mixture of acetonitrile (3.2), sodium dihydrogen phosphate solution (3.4) and sodium perchlorate solution (3.5), 450+450+100 (v+v+v).
Flow rate:
0,7-1 ml/min
Detection wavelength:
264 nm
Injection volume:
100 μl
Check the stability of the chromatographic system, injecting several times the calibration solution (3.7.3) containing 1,0 μg/ml, until constant peak heights and retention times are achieved.

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/VIII/division/C/division/5/division/5.3/division/5.3.1 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-VIII/division-C/division-5/division-5.3/division-5.3.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.