# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 3.11.2: Calibration solutions

Extent: E+W+S+N.I.

Transfer 2,0, 5,0, 10,0, and 20,0 ml of the stock standard solution (3.11.1) into a series of 100 ml calibrated flasks. Add 30 ml of water, make up to the mark with methanol-acetonitrile (3.5) and mix. Wrap the flasks with aluminium foil. These solutions correspond to 2,0, 5,0, 10,0 and 20,0 μg/ml of carbadox respectively.
Calibration solutions must be freshly prepared before use.
Note:For the determination of carbadox in feed containing less than 10 mg/kg, calibration solutions with a concentration below 2,0 μg/ml must be prepared.

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/VIII/division/D/division/3/division/3.11.2 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-VIII/division-D/division-3/division-3.11.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.