# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 5.2.2: Premixtures (0,1 %-2,0 %)

Extent: E+W+S+N.I.

Weigh to the nearest 0,001 g, 1 g of the unground sample and transfer to a 200 ml conical flask. Add 15,0 ml of water, mix, and equilibrate for 5 min. Add 35,0 ml of methanol-acetonitrile (3.5), stopper and shake for 30 min. on the shaker or stir on the magnetic stirrer (4.1). Filter the solution through a glass fibre filter paper (4.2).
Pipet an aliquot of filtrate into a 50 ml calibrated flask. Add 15,0 ml of water, make up to the mark with methanol-acetonitrile (3.5) and mix. The carbadox concentration of the final solution shall be approximately 10 μg/ml. An aliquot is filtered through a 0,45 μm filter (4.6).
Proceed to the HPLC determination (5.4).

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/VIII/division/D/division/5/division/5.2/division/5.2.2 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-VIII/division-D/division-5/division-5.2/division-5.2.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.