# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 5.2.3: Preparations (> 2 %)

Extent: E+W+S+N.I.

Weigh to the nearest 0,001 g, 0,2 g of the unground sample and transfer to a 250 ml conical flask. Add 45,0 ml of water, mix, and equilibrate for 5 min. Add 105,0 ml of methanol-acetonitrile (3.5), stopper and homogenise. Sonicate (4.7) the sample for 15 min. followed by shaking or stirring for 15 min. (4.1). Filter the solution through a glass fibre filter paper (4.2).
Dilute an aliquot of filtrate with the mixture of water-methanol-acetonitrile (3.12) to a final carbadox concentration of 10-15 μg/ml (for a 10 % preparation, the dilution factor is 10). An aliquot is filtered through a 0,45 μm filter (4.6).
Proceed to the HPLC determination (5.4).

---
Source: https://www.legislation.gov.uk/eur/2009/152/annex/VIII/division/D/division/5/division/5.2/division/5.2.3 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-VIII/division-D/division-5/division-5.2/division-5.2.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.