{
  "url": "https://openlegislation.uk/eu-regulation-2009-152/annex-VIII/division-D/division-5/division-5.4/division-5.4.1",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-152/annex-VIII/division-D/division-5/division-5.4/division-5.4.1.json",
  "legislation": "eur/2009/152",
  "legislation_title": "Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-152",
  "path": "annex/VIII/division/D/division/5/division/5.4/division/5.4.1",
  "kind": "division",
  "number": "5.4.1",
  "heading": "Parameters",
  "text": "The following conditions are offered for guidance, other conditions may be used provided they yield equivalent results:\nLiquid chromatographic\ncolumn (4.4.1):\n300 mm × 4 mm, C18, 10 μmpacking or equivalent\nMobile phase (3.10):\nMixture of acetate buffer solution (3.9) and acetonitrile (3.2), 825 + 175 (v+v)\nFlow rate:\n1,5-2 ml/min.\nDetection wavelength:\n365 nm\nInjection volume:\n20 μl\nCheck the stability of the chromatographic system, injecting the calibration solution (3.11.2) containing 5,0 μg/ml several times, until constant peak heights (areas) and retention times are achieved.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/152/annex/VIII/division/D/division/5/division/5.4/division/5.4.1",
    "retrieved_at": "2026-11-01T14:51:38.610873+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}