# Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed (Text with EEA relevance), Division 5.4.1: Parameters

Extent: E+W+S+N.I.

The following conditions are offered for guidance, other conditions may be used provided they yield equivalent results:
Liquid chromatographic
column (4.4.1):
300 mm × 4 mm, C18, 10 μmpacking or equivalent
Mobile phase (3.10):
Mixture of acetate buffer solution (3.9) and acetonitrile (3.2), 825 + 175 (v+v)
Flow rate:
1,5-2 ml/min.
Detection wavelength:
365 nm
Injection volume:
20 μl
Check the stability of the chromatographic system, injecting the calibration solution (3.11.2) containing 5,0 μg/ml several times, until constant peak heights (areas) and retention times are achieved.

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Source: https://www.legislation.gov.uk/eur/2009/152/annex/VIII/division/D/division/5/division/5.4/division/5.4.1 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-152/annex-VIII/division-D/division-5/division-5.4/division-5.4.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.