{
  "url": "https://openlegislation.uk/eu-regulation-2009-761/annex-IV/division-1/division-1.8/division-1.8.4",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-761/annex-IV/division-1/division-1.8/division-1.8.4.json",
  "legislation": "eur/2009/761",
  "legislation_title": "Commission Regulation (EC) No 761/2009 of 23 July 2009 amending, for the purpose of its adaptation to technical progress, Regulation (EC) No 440/2008 laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-761",
  "path": "annex/IV/division/1/division/1.8/division/1.8.4",
  "kind": "division",
  "number": "1.8.4",
  "heading": "Initial biomass concentration",
  "text": "The initial biomass in the test cultures must be the same in all test cultures and sufficiently low to allow exponential growth throughout the incubation period without risk of nutrient depletion. The initial biomass should not exceed 0,5 mg/l as dry weight. The following initial cell concentrations are recommended:\nPseudokirchneriella subcapitata\n5 × 103-104\ncells/ml\nDesmodesmus subspicatus\n2-5 × 103\ncells/ml\nNavicula pelliculosa\n104\ncells/ml\nAnabaena flos-aquae\n104\ncells/ml\nSynechococcus leopoliensis\n5 × 104-105\ncells/ml",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/761/annex/IV/division/1/division/1.8/division/1.8.4",
    "retrieved_at": "2026-11-01T14:51:19.625001+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}