# Commission Regulation (EC) No 761/2009 of 23 July 2009 amending, for the purpose of its adaptation to technical progress, Regulation (EC) No 440/2008 laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (Text with EEA relevance), Division 1.8.6: Replicates and controls

Extent: E+W+S+N.I.

The test design should include three replicates at each test concentration. If determination of the NOEC is not required, the test design may be altered to increase the number of concentrations and reduce the number of replicates per concentration. The number of control replicates must be at least three, and ideally should be twice the number of replicates used for each test concentration.
A separate set of test solutions may be prepared for analytical determinations of test substance concentrations (see the fourth and sixth paragraphs in section 1.8.11).
When a solvent is used to solubilise the test substance, additional controls containing the solvent at the same concentration as used in the test cultures must be included in the test design.

---
Source: https://www.legislation.gov.uk/eur/2009/761/annex/IV/division/1/division/1.8/division/1.8.6 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-761/annex-IV/division-1/division-1.8/division-1.8.6
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.