# Commission Regulation (EC) No 761/2009 of 23 July 2009 amending, for the purpose of its adaptation to technical progress, Regulation (EC) No 440/2008 laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (Text with EEA relevance), Division 1.8.8: Preparation of test solutions

Extent: E+W+S+N.I.

All test solutions must contain the same concentrations of growth medium and initial biomass of test algae. Test solutions of the chosen concentrations are usually prepared by mixing a stock solution of the test substance with growth medium and inoculum culture. Stock solutions are normally prepared by dissolving the substance in test medium.
Solvents, e.g. acetone, t-butyl alcohol and dimethyl formamide, may be used as carriers to add substances of low water solubility to the test medium (2)(3). The solvent concentration should not exceed 100 µl/l, and the same concentration of solvent should be added to all cultures (including controls) in the test series.

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Source: https://www.legislation.gov.uk/eur/2009/761/annex/IV/division/1/division/1.8/division/1.8.8 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-761/annex-IV/division-1/division-1.8/division-1.8.8
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.