{
  "url": "https://openlegislation.uk/eu-regulation-2009-761/annex-IV/division-3/division-3.1",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-761/annex-IV/division-3/division-3.1.json",
  "legislation": "eur/2009/761",
  "legislation_title": "Commission Regulation (EC) No 761/2009 of 23 July 2009 amending, for the purpose of its adaptation to technical progress, Regulation (EC) No 440/2008 laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-761",
  "path": "annex/IV/division/3/division/3.1",
  "kind": "division",
  "number": "3.1",
  "heading": "TEST REPORT",
  "text": "The test report must include the following:\nTest substance:\nphysical nature and relevant physiochemical properties, including water solubility limit,\nchemical identification data, including purity.\nTest species:\nthe strain, supplier or source and the culture conditions used.\nTest conditions:\ndate of start of the test and its duration,\ndescription of test design: test vessels, culture volumes, biomass density at the beginning of the test,\ncomposition of the medium,\ntest concentrations and replicates (e.g. number of replicates, number of test concentrations and geometric progression used),\ndescription of the preparation of test solutions, including use of solvents etc.,\nculturing apparatus,\nlight intensity and quality (source, homogeneity),\ntemperature,\nconcentrations tested: the nominal test concentrations and any results of analyses to determine the concentration of the test substance in the test vessels. The recovery efficiency of the method and the limit of quantification in the test matrix should be reported,\nall deviations from this Testing Method,\nmethod for determination of biomass and evidence of correlation between the measured parameter and dry weight.\nResults:\npH values at the start and end of the test at all treatments,\nbiomass for each flask at each measuring point and method for measuring biomass,\ngrowth curves (plot of biomass versus time),\ncalculated response variables for each treatment replicate, with mean values and coefficient of variation for replicates,\ngraphical presentation of the concentration/effect relationship,\nestimates of toxicity for response variables e.g. EC50, EC10, EC20 and associated confidence intervals. If calculated, LOEC and NOEC and the statistical methods used for their determination,\nif ANOVA has been used, the size of the effect which can be detected (e.g. the least significant difference),\nany stimulation of growth found in any treatment,\nany other observed effects, e.g. morphological changes of the algae,\ndiscussion of the results, including any influence on the outcome of the test resulting from deviations from this Testing Method.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/761/annex/IV/division/3/division/3.1",
    "retrieved_at": "2026-11-01T14:51:19.625001+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}