# Commission Regulation (EC) No 761/2009 of 23 July 2009 amending, for the purpose of its adaptation to technical progress, Regulation (EC) No 440/2008 laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (Text with EEA relevance), Division 3.1: TEST REPORT

Extent: E+W+S+N.I.

The test report must include the following:
Test substance:
physical nature and relevant physiochemical properties, including water solubility limit,
chemical identification data, including purity.
Test species:
the strain, supplier or source and the culture conditions used.
Test conditions:
date of start of the test and its duration,
description of test design: test vessels, culture volumes, biomass density at the beginning of the test,
composition of the medium,
test concentrations and replicates (e.g. number of replicates, number of test concentrations and geometric progression used),
description of the preparation of test solutions, including use of solvents etc.,
culturing apparatus,
light intensity and quality (source, homogeneity),
temperature,
concentrations tested: the nominal test concentrations and any results of analyses to determine the concentration of the test substance in the test vessels. The recovery efficiency of the method and the limit of quantification in the test matrix should be reported,
all deviations from this Testing Method,
method for determination of biomass and evidence of correlation between the measured parameter and dry weight.
Results:
pH values at the start and end of the test at all treatments,
biomass for each flask at each measuring point and method for measuring biomass,
growth curves (plot of biomass versus time),
calculated response variables for each treatment replicate, with mean values and coefficient of variation for replicates,
graphical presentation of the concentration/effect relationship,
estimates of toxicity for response variables e.g. EC50, EC10, EC20 and associated confidence intervals. If calculated, LOEC and NOEC and the statistical methods used for their determination,
if ANOVA has been used, the size of the effect which can be detected (e.g. the least significant difference),
any stimulation of growth found in any treatment,
any other observed effects, e.g. morphological changes of the algae,
discussion of the results, including any influence on the outcome of the test resulting from deviations from this Testing Method.

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Source: https://www.legislation.gov.uk/eur/2009/761/annex/IV/division/3/division/3.1 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-761/annex-IV/division-3/division-3.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.