# Commission Regulation (EC) No 761/2009 of 23 July 2009 amending, for the purpose of its adaptation to technical progress, Regulation (EC) No 440/2008 laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (Text with EEA relevance), Division 1.3: APPLICABILITY OF THE TEST

Extent: E+W+S+N.I.

This simulation test is applicable to non-volatile or slightly volatile organic substances tested at low concentrations. Using flasks open to the atmosphere (e.g. cotton wool plugged), substances with Henry’s law constants less than about 1 Pa·m3/mol (approx. 10–5 atm·m3/mol) can be regarded as non-volatile in practice. Using closed flasks with a headspace, it is possible to test slightly volatile substances (with Henry’s law constants < 100 Pa·m3/mol or < 10–3 atm·m3/mol) without losses from the test system. Loss of 14C-labelled substances may occur, if the right precautions are not exercised, when the CO2 is stripped off. In such situations, it may be necessary to trap CO2 in an internal absorber with alkali or to use an external CO2 absorber system (direct 14CO2 determination; see Appendix 3). For the determination of biodegradation kinetics, the concentrations of the test substance must be below its water solubility. It should be noted, however, that literature values of water solubility may be considerably higher than the solubility of the test substance in natural waters. Optionally, the solubility of especially poorly water-soluble test substances may be established by use of the natural waters being tested.
The method can be used for simulating biodegradation in surface water free of coarse particles (pelagic test) or in turbid surface water which, e.g. might exist near a water/sediment interface (suspended sediment test).

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Source: https://www.legislation.gov.uk/eur/2009/761/annex/V/division/1/division/1.3 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-761/annex-V/division-1/division-1.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.