OpenLegislation

Division 3 TEST REPORT

United Kingdom

Original text. Later amendments are not shown.

The type of study, i.e. pelagic or suspended sediment test, must be clearly stated in the test report, which shall also contain at least the following information:

Test substance and reference substance(s):

  • common names, chemical names (recommend IUPAC and/or CAS names), CAS numbers, structural formulas (indicating position of 14C if radiolabelled substance is used) and relevant physico-chemical properties of test and reference substance (see sections 1.5 and 1.6),

  • chemical names, CAS numbers, structural formulas (indicating position of 14C if radiolabelled substance is used) and relevant physico-chemical properties of substances used as standards for identification and quantification of transformation products,

  • purity (impurities) of test and reference substances,

  • radiochemical purity of labelled chemical and specific activity (where appropriate).

Surface water:

The following minimum information for the water sample taken must be provided:

  • location and description of sampling site including, if possible, contamination history,

  • date and time of sample collection,

  • nutrients (total N, ammonium, nitrite, nitrate, total P, dissolved orthophosphate),

  • depth of collection,

  • appearance of sample (e.g. colour and turbidity),

  • DOC and TOC,

  • BOD,

  • temperature and pH at the place and time of collection,

  • oxygen or redox potential (mandatory only if aerobic conditions are not obvious),

  • salinity or conductivity (in the case of sea water and brackish water),

  • suspended solids (in case of a turbid sample),

  • possibly other relevant information about the sampling location at the time of sampling (e.g. actual or historical data on flow rate of rivers or marine currents, nearby major discharges and type of discharges, weather conditions preceding the sampling time),

and optionally:

  • microbial biomass (e.g. acridine orange direct count or colony forming units),

  • inorganic carbon,

  • chlorophyll-a concentration as a specific estimate for algal biomass.

In addition, the following information on the sediment should be provided if the suspended sediment test is conducted:

  • depth of sediment collection,

  • appearance of the sediment (such as coloured, muddy, silty, or sandy),

  • texture (e.g. % coarse sand, fine sand, silt and clay),

  • dry weight in g/l of the suspended solids, TOC concentration or weight loss on ignition as a measure of the content of organic matter,

  • pH,

  • oxygen or redox potential (mandatory only if aerobic conditions are not obvious).

Test conditions:

  • delay between collection and use in the laboratory test, sample storage and pre-treatment of the sample, dates of performance of the studies,

  • amount of test substance applied, test concentration and reference substance,

  • method of application of the test substance including any use of solvents,

  • volume of surface water used and sediment (if used) and volume sampled at each interval for analysis,

  • description of the test system used.

If dark conditions are not to be maintained, information on the ‘diffuse light’ conditions:

  • information on the method(s) used for establishing sterile controls (e.g. temperature, time and number of autoclavings),

  • incubation temperature,

  • information on analytical techniques and the method(s) used for radiochemical measurements and for mass balance check and measurements of phase distribution (if conducted),

  • number of replicates.

Results:

  • percentages of recovery (see section 1.7.1),

  • repeatability and sensitivity of the analytical methods used including the limit of detection (LOD) and the limit of quantification (LOQ) (see section 1.7.2),

  • all measured data (including sampling time points) and calculated values in tabular form and the degradation curves; for each test concentration and for each replicate flask, report the linear correlation coefficient for the slope of the logarithmic plot, the estimated lag phase and a first-order or pseudo-first order rate constant (if possible), and the corresponding degradation half-life (or the half-life period, t50),

  • report relevant values as the averages of the results observed in individual replicates, e.g. length of lag phase, degradation rate constant and degradation half-life (or t50),

  • categorise the system as either non-adapted or adapted as judged from the appearance of the degradation curve and from the possible influence of the test concentration,

  • the results of the final mass balance check and results on phase distribution measurements (if any),

  • the fraction of 14C mineralised and, if specific analyses are used, the final level of primary degradation,

  • the identification, molar concentration and percentage of applied and major transformation products (see first paragraph in section 1.8.9.5), where appropriate,

  • a proposed pathway of transformation, where appropriate,

  • discussion of results.