# Commission Regulation (EC) No 761/2009 of 23 July 2009 amending, for the purpose of its adaptation to technical progress, Regulation (EC) No 440/2008 laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (Text with EEA relevance), Division 1.7.3: Cultivation

Extent: E+W+S+N.I.

To reduce the frequency of culture maintenance (e.g. when no Lemna tests are planned for a period), cultures can be held under reduced illumination and temperature (4-10 °C). Details of culturing are given in Appendix 2. Obvious signs of contamination by algae or other organisms will require surface sterilisation of a sub-sample of Lemna fronds, followed by transfer to fresh medium (see Appendix 2). In this eventuality, the remaining contaminated culture should be discarded.
At least seven days before testing, sufficient colonies are transferred aseptically into fresh sterile medium and cultured for 7-10 days under the conditions of the test.

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Source: https://www.legislation.gov.uk/eur/2009/761/annex/VI/division/1/division/1.7/division/1.7.3 (the official version)
Page: https://openlegislation.uk/eu-regulation-2009-761/annex-VI/division-1/division-1.7/division-1.7.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.