{
  "url": "https://openlegislation.uk/eu-regulation-2009-761/annex-VI/division-3/division-3.1",
  "json_url": "https://openlegislation.uk/eu-regulation-2009-761/annex-VI/division-3/division-3.1.json",
  "legislation": "eur/2009/761",
  "legislation_title": "Commission Regulation (EC) No 761/2009 of 23 July 2009 amending, for the purpose of its adaptation to technical progress, Regulation (EC) No 440/2008 laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2009-761",
  "path": "annex/VI/division/3/division/3.1",
  "kind": "division",
  "number": "3.1",
  "heading": "TEST REPORT",
  "text": "The test report must include the following:\nTest substance:\nphysical nature and physicochemical properties, including water solubility limit,\nchemical identification data (e.g. CAS Number), including purity.\nTest species:\nscientific name, clone (if known) and source.\nTest conditions:\ntest procedure used (static, semi-static or flow-through),\ndate of start of the test and its duration,\ntest medium,\ndescription of the experimental design: test vessels and covers, solution volumes, number of colonies and fronds per test vessel at the beginning of the test,\ntest concentrations (nominal and measured as appropriate) and number of replicates per concentration,\nmethods of preparation of stock and test solutions including the use of any solvents or dispersants,\ntemperature during the test,\nlight source, light intensity and homogeneity,\npH values of the test and control media,\ntest substance concentrations and the method of analysis with appropriate quality assessment data (validation studies, standard deviations or confidence limits of analyses),\nmethods for determination of frond number and other measurement variables, e.g. dry weight, fresh weight or frond area,\nall deviations from this Testing Method.\nResults:\nraw data: number of fronds and other measurement variables in each test and control vessel at each observation and occasion of analysis,\nmeans and standard deviations for each measurement variable,\ngrowth curves for each concentration (recommended with log transformed measurement variable, see second paragraph in section 2.2.1),\ndoubling time/growth rate in the control based on the frond number,\ncalculated response variables for each treatment replicate, with mean values and coefficient of variation for replicates,\ngraphical representation of the concentration/effect relationship,\nestimates of toxic endpoints for response variables e.g. EC50, EC10, EC20, and associated confidence intervals. If calculated, LOEC and/or NOEC and the statistical methods used for their determination,\nif ANOVA has been used, the size of the effect which can be detected (e.g. the least significant difference),\nany stimulation of growth found in any treatment,\nany visual signs of phytotoxicity as well as observations of test solutions,\ndiscussion of the results, including any influence on the outcome of the test resulting from deviations from this Testing Method.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2009/761/annex/VI/division/3/division/3.1",
    "retrieved_at": "2026-11-01T14:51:19.625001+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}