{
  "url": "https://openlegislation.uk/eu-regulation-2010-175/annex-I/part-B/division-4/division-4.2/division-4.2.3",
  "json_url": "https://openlegislation.uk/eu-regulation-2010-175/annex-I/part-B/division-4/division-4.2/division-4.2.3.json",
  "legislation": "eur/2010/175",
  "legislation_title": "Commission Regulation (EU) No 175/2010 of 2 March 2010 implementing Council Directive 2006/88/EC as regards measures to control increased mortality in oysters of the species Crassostrea gigas in connection with the detection of Ostreid herpesvirus 1 μvar (OsHV-1 μvar) (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2010-175",
  "path": "annex/I/part/B/division/4/division/4.2/division/4.2.3",
  "kind": "division",
  "number": "4.2.3",
  "heading": "PCR mix",
  "text": "PCR mix for each tube is:\nVolume per tube\nFinal concentration\nBuffer (10 X)\n5 μl\n1 X\nMgCl2 (25 mM)\n5 μl\n2,5 mM\ndNTP (2 mM)\n5 μl\n0,2 mM\nCF (10 μM)\n1 μl\n0,2 μM\nCR (10 μM)\n1 μl\n0,2 μM\nTaq polymérase (5U/μl)\n0,5 μl\n2,5 U\ndH2O\n31,5 μl\n49 μl of this PCR mix is dispensed in each PCR tube\n1 μl of extracted DNA (50-100 ng/μl) is added to each tube",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2010/175/annex/I/part/B/division/4/division/4.2/division/4.2.3",
    "retrieved_at": "2026-11-01T14:50:54.654314+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}